Sephadex™ LH-20

Technical Informations

Matrix…………………………………. ....Hydroxypropylated, cross-linked dextran 

Bead form…………………………….......Spherical, porous 

Particle size range (diameter)   

Dry…………………………………………18-111 µm 

in methanol…………………………….....27-163 µm 

pH stability ……………………………....2-13 (working and cleaning) 

Chemical stability…………………........Stable in most aqueous and organic eluent systems. Notstable below pH 2 nor to strong oxidising agents. 

Autoclavable…………………………......20 min at 121°C 

Maximum linear flow rate………...........~12 cm/min 

Recommended linear flow rate…..........~1 cm/min but depends on resolution required 

Operating temperature……………….....4°C to 40°C 

Sample loading volumes   

adsorption mode………………………...determined by desired degree of resolution 

Molecular sizing…………………………~2% total bed volume 

Partition mode…………………………..~1% total bed volume 

Exclution limit*…………………………..peptides ~4 kDa, small organics ~5 kDa 

Shelf-life…………………………………..5 years 

Recommended storage………………....Maintain dry at 4°C to 25°C, wet 4°C to +8°C, pH 6-8, with bacteriostat