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Pepsin 1:10000 胃蛋白酶1:10000

所属分类:酶和辅酶
商品编号:P7000
商品规格:10g分装
计量单位:
库存现状:50
参考价:¥180
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商品简介

详细说明

产品编号 产品名称  规格  价格  备注
P7000-10 Pepsin 1:10000 胃蛋白酶1:10000  10g 180.00 Sigma分装
P7000-25 Pepsin 1:10000 胃蛋白酶1:10000  25g 380.00 Sigma分装

产品详细说明:

Pepsin from porcine gastric mucosa

Synonym : Pepsin A

Pepsin from hog stomach

CAS Number 9001-75-6

(EC) Number: 3.4.23.1

Descriptions:

Analysis Note: E1%/280=14.7

Protein determined by E1%/280

Application: Used to produce F(ab ′ )2 fragments of antibodies.

Biochem/physiol Actions: Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at

Val, Ala or Gly. Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine

HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH>6.

Unit Definition: One unit will produce a ΔA 280 of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.)

Properties:

form ………………………………………………………………powder

mol wt……………………………………………………………mol wt 35 kDa

Gene Information ………………………………………………pig ... LOC396892(396892)

Risk Statements: 36/37/38-42

Safety Statements: 22-24-26-36/37

TEST

SPECIFICATION

APPEARANCE

REPORT RESULT

SOLUBILITY

CLEAR TO SLIGHTLY HAZY COLORLESS SOLUTION AT 1 MG/ML IN 0.01N HCL

PROTEIN BY UV ABSORBANCE

REPORT RESULT

LOSS ON DRYING

NMT 5%

ENZYMATIC ACTIVITY

800 TO 2500 UNITS/MG PROTEIN

UNIT DEFINITION

ONE UNIT WILL PRODUCE A CHANGE IN A280 OF 0.001 PER MINUTE AT PH2.0 AT 37DEGC, MEASURED AS TCA-SOLUBLE PRODUCTS USING HEMOGLOBIN AS SUBSTRATE. (FINAL VOLUME = 16ML. LIGHTPATH=1CM.)

RECOMMENDED RETEST

2 YEARS

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